Crystal structures of adenine phosphoribosyltransferase from Leishmania donovani
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چکیده
منابع مشابه
Crystal structures of adenine phosphoribosyltransferase from Leishmania donovani.
The enzyme adenine phosphoribosyltransferase (APRT) functions to salvage adenine by converting it to adenosine-5-monophosphate (AMP). APRT deficiency in humans is a well characterized inborn error of metabolism, and APRT may contribute to the indispensable nutritional role of purine salvage in protozoan parasites, all of which lack de novo purine biosynthesis. We determined crystal structures f...
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a Department of Molecular, Cell and De6elopmental Biology, Howard Hughes Medical Institute, UCLA School of Medicine, UCLA, Los Angeles, CA 90095-1662, USA b Howard Hughes Medical Institute, UCLA School of Medicine, UCLA, Los Angeles, CA 90095-1662, USA c Department of Medical Microbiology, Immunology and Molecular Genetics, Howard Hughes Medical Institute, UCLA School of Medicine, UCLA, Los Ang...
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The structural requirements for the binding of purines to adenine phosphoribosyltransferase from monkey liver were explored by the determination of the K; values for 67 purines and purine analogues. Adenine was bound 40 times better than the most effectively bound analogues. Substitutions on the 6-amino group of adenine decreased binding to varying degrees. The hydroxylaminoand hydrazinopurines...
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The subcellular location of a protein is a critical factor in its physiological function and an important consideration in therapeutic paradigms that target the protein. Because Leishmania donovani cannot synthesize purine nucleotides de novo, they rely predominantly upon therapeutically germane phosphoribosyltransferase (PRT) enzymes, hypoxanthine-guanine PRT (HGPRT), adenine PRT (APRT), and x...
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ژورنال
عنوان ژورنال: The EMBO Journal
سال: 1999
ISSN: 1460-2075
DOI: 10.1093/emboj/18.13.3533